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Accumulation of large amounts of intracellularK+ in astrocytes decreases local extracellular K+ gradients.The astrocyte membrane becomes depolarized, and thecharge is dissipated over a large area by the extensive networkofastrocyte processes. This histochemical method demonstrates and local-izes carbohydrates and carbohydrate-rich macromolecules This histochemical method demonstrates and local-izes carbohydrates and carbohydrate-rich macromolecules.